This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-AU1-mTOR-E2419K
catalog :
19994
citations: 6
Reference
Booth L, West C, Von Hoff D, Kirkwood J, Dent P. GZ17-6.02 Interacts With [MEK1/2 and B-RAF Inhibitors] to Kill Melanoma Cells. Front Oncol. 2021;11:656453 pubmed publisher
Li S, Song Y, Quach C, Guo H, Jang G, Maazi H, et al. Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun. 2019;10:1693 pubmed publisher
Rajala A, Wang Y, Rajala R. Constitutive Activation Mutant mTOR Promote Cone Survival in Retinitis Pigmentosa Mice. Adv Exp Med Biol. 2018;1074:491-497 pubmed publisher
Okada K, Nogami A, Ishida S, Akiyama H, Chen C, Umezawa Y, et al. FLT3-ITD induces expression of Pim kinases through STAT5 to confer resistance to the PI3K/Akt pathway inhibitors on leukemic cells by enhancing the mTORC1/Mcl-1 pathway. Oncotarget. 2018;9:8870-8886 pubmed publisher
Palmieri M, Pal R, Nelvagal H, Lotfi P, Stinnett G, Seymour M, et al. mTORC1-independent TFEB activation via Akt inhibition promotes cellular clearance in neurodegenerative storage diseases. Nat Commun. 2017;8:14338 pubmed publisher
Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F. Point mutations in TOR confer Rheb-independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells. Proc Natl Acad Sci U S A. 2007;104:3514-9 pubmed
product information
Catalog Number :
19994
Product Name :
pcDNA3-AU1-mTOR-E2419K
article :
doi10.1073/pnas.0608510104
id2082
pubmed_id17360675
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
mTOR-E2419K
cloning
clone_methodRestriction Enzyme
cloning_site_3Not I
cloning_site_5Hind III
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFrap1, RAFT1
geneMtor
id56718
genbank_ids
mutationChanged Glutamine 2419 to Lysine
namemammalian target of rapamycin-E2419K
shRNA_sequence
size7700
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagAU1
resistance markers :
517
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA