This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hPU.1 N165-pET28b
catalog :
199796
citations: 1
Reference
Xhani S, Lee S, Kim H, Wang S, Esaki S, Ha V, et al. Intrinsic disorder controls two functionally distinct dimers of the master transcription factor PU.1. Sci Adv. 2020;6:eaay3178 pubmed publisher
product information
Catalog Number :
199796
Product Name :
hPU.1 N165-pET28b
article :
doi10.1126/sciadv.aay3178
id28234095
pubmed_id32128405
bacterial resistance :
Kanamycin
cloning :
backbonepET28b
backbone_mutation
backbone_originNovagen
backbone_size5246
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli
growth temp :
Induce with 0.5 mM IPTG at 30 C for protein expression.
origin :
37
pi :
alt_names
Spi-1 proto-oncogene
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NcoI
promoter
sequencing_primer_3T7terminator
sequencing_primer_5T7promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAGM10, OF, PU.1, SFPI1, SPI-1, SPI-A
geneSPI1
id6688
genbank_ids
mutation
namespi1
shRNA_sequence
size326
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagNone
resistance markers :
3167
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA