This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC2-gephyrin C4a
catalog :
199611
citations: 1
Reference
Campbell B, Dittmann A, Dreier B, Pl xfc ckthun A, Tyagarajan S. A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology. elife. 2022;11: pubmed publisher
product information
Catalog Number :
199611
Product Name :
pEGFPC2-gephyrin C4a
article :
doi10.7554/eLife.80895
id28234062
pubmed_id36314779
bacterial resistance :
Kanamycin
cloning :
backbonepEGFPC2
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Gephyrin C4a cassette encodes ARLPSCSSTYSVSE after K288 (expressed in neurons and promotes oligomerization of gephyrin molecules for synapse scaffolding. Inhibitory synapse distribution for C4a and P1 variant of gephyrin currently unknown)
growth strain :
Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGeph
geneGphn
id64845
genbank_ids
mutationC4a casette insertion
nameGephyrin
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
2515
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA