This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC2-gephyrin C3
catalog :
199610
citations: 1
Reference
Campbell B, Dittmann A, Dreier B, Pl xfc ckthun A, Tyagarajan S. A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology. elife. 2022;11: pubmed publisher
product information
Catalog Number :
199610
Product Name :
pEGFPC2-gephyrin C3
article :
doi10.7554/eLife.80895
id28234062
pubmed_id36314779
bacterial resistance :
Kanamycin
cloning :
backbonepEGFPC2
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Gephyrin C3 cassette encodes NHPFYTSPAVFMANHGQPIPGLISYSHHATGSADKR after K243 (Thought to be expressed in astrocytes for MoCo biosynthesis and prevents olgomerization of gephyrin molecules for synapse scaffolding function in neurons)
growth strain :
Encodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGeph
geneGphn
id64845
genbank_ids
mutationC3 cassette insertion
nameGephyrin
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
2515
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA