pcDNA3-HA-CUL1 Y42A/M43A | Addgene 19940 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-HA-CUL1 Y42A/M43A

catalog :

19940

citations: 2

Reference
Mudhasani R, Tran J, Retterer C, Kota K, Whitehouse C, Bavari S. Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase. PLoS Pathog. 2016;12:e1005437 pubmed publisher
Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y. The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. Mol Cell Biol. 2000;20:8185-97 pubmed

product information

Catalog Number :

19940

Product Name :

pcDNA3-HA-CUL1 Y42A/M43A

article :

doi	10.1128/MCB.20.21.8185-8197.2000
id	2352
pubmed_id	11027288

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3-HA

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

flag tag is a variant: YPYDLPDYA S2A mutation present but does not appear to affect function

origin :

pi :

alt_names

CUL1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	CUL1
id	8454

genbank_ids

NP_003583

mutation

CUL1 with a SKP1 binding mutant

name

cullin 1 Y42A/M43A

shRNA_sequence

size

2260

species

9606

Homo sapiens