This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUt-mNF-BACH1 Cas9-Resistant
catalog :
199218
citations: 1
Reference
Wan Y, Cohen J, Szenk M, Farquhar K, Coraci D, Krzyszto x144 R, et al. Nonmonotone invasion landscape by noise-aware control of metastasis activator levels. Nat Chem Biol. 2023;19:887-899 pubmed publisher
product information
Catalog Number :
199218
Product Name :
pUt-mNF-BACH1 Cas9-Resistant
article :
doi10.1038/s41589-023-01344-z
id28233996
pubmed_id37231268
bacterial resistance :
Blasticidin
cloning :
backbonepUt-mNF-BACH1
backbone_mutation
backbone_originBalazsi Lab
backbone_size7717
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection.
origin :
37
pi :
alt_names
Tetracycline Repressor codon-optimized for human
BTB Domain And CNC Homolog 1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV D2ir promoter
sequencing_primer_3GATGAGTAAACCGGTGCGGC
sequencing_primer_5ATGTCTCTGAGTGAGAACTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBACH-1, BTBD24
geneBACH1
id571
genbank_ids
NM_206866
mutationsilient mutation at nucleic acid 177 C changed to T
namehTetR::P2A::EGFP::BACH1
shRNA_sequence
size3657
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
1506
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA