This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-hMICU1EF
catalog :
199180
citations: 1
Reference
Tsai C, Liu T, Chao F, Tu Y, Rodriguez M, Van Keuren A, et al. Evidence supporting the MICU1 occlusion mechanism and against the potentiation model in the mitochondrial calcium uniporter complex. Proc Natl Acad Sci U S A. 2023;120:e2217665120 pubmed publisher
product information
Catalog Number :
199180
Product Name :
pET-hMICU1EF
article :
doi10.1073/pnas.2217665120
id28233984
pubmed_id37036971
bacterial resistance :
Kanamycin
cloning :
backbonepET24
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth strain :
This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesCALC, CBARA1, EFHA3, MPXPS, ara CALC
geneMICU1
id10367
genbank_ids
mutationD421A, E432K, F433W, F453W
nameMICU1
shRNA_sequence
size2450
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHis6
locationN terminal on insert
tagMBP, Thrombin site, TEV site
resistance markers :
5725
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA