This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-FLAG-DDB1
catalog :
19918
citations: 12
Reference
Houles T, Boucher J, Lavoie G, Macleod G, Lin S, Angers S, et al. The CDK12 inhibitor SR-4835 functions as a molecular glue that promotes cyclin K degradation in melanoma. Cell Death Discov. 2023;9:459 pubmed publisher
Mayor Ruiz C, Bauer S, Brand M, Kozicka Z, Siklos M, Imrichová H, et al. Rational discovery of molecular glue degraders via scalable chemical profiling. Nat Chem Biol. 2020;: pubmed publisher
Fu S, Hu M, Peng Y, Fang H, Hsiao C, Chen T, et al. CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy. Cells. 2020;9: pubmed publisher
Nowak M, Suenkel B, Porras P, Migotti R, Schmidt F, Kny M, et al. DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy. J Cell Sci. 2019;132: pubmed publisher
Lu G, Yi J, Gubas A, Wang Y, Wu Y, Ren Y, et al. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation. Autophagy. 2019;:1-18 pubmed publisher
Landsberg C, Megger D, Hotter D, Rückborn M, Eilbrecht M, Rashidi Alavijeh J, et al. A Mass Spectrometry-Based Profiling of Interactomes of Viral DDB1- and Cullin Ubiquitin Ligase-Binding Proteins Reveals NF-κB Inhibitory Activity of the HIV-2-Encoded Vpx. Front Immunol. 2018;9:2978 pubmed publisher
Zhou X, Shi H, Yang S, Sun P. An efficient rapid system for assaying HBx-mediated transactivation. Biotechnol Lett. 2017;39:1091-1099 pubmed publisher
Brown M, Gromeier M. MNK Controls mTORC1:Substrate Association through Regulation of TELO2 Binding with mTORC1. Cell Rep. 2017;18:1444-1457 pubmed publisher
Luo Y, Jacobs E, Greco T, Mohammed K, Tong T, Keegan S, et al. HIV-host interactome revealed directly from infected cells. Nat Microbiol. 2016;1:16068 pubmed
Chen Y, Peng Y, Hu M, Huang J, Chien Y, Wu J, et al. The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels. Sci Rep. 2015;5:10667 pubmed publisher
Wu Z, Chen Y, Yang T, Gao Q, Yuan M, Ma L. Targeted ubiquitination and degradation of G-protein-coupled receptor kinase 5 by the DDB1-CUL4 ubiquitin ligase complex. PLoS ONE. 2012;7:e43997 pubmed publisher
Hu J, Zacharek S, He Y, Lee H, Shumway S, Duronio R, et al. WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. Genes Dev. 2008;22:866-71 pubmed publisher
product information
Catalog Number :
19918
Product Name :
pcDNA3-FLAG-DDB1
article :
doi10.1101/gad.1624008
id2346
pubmed_id18381890
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5473
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addgene QC next-generation sequencing found that the insert encodes a Leu as the terminal amino acid, rather than His found in NM_001923.
origin :
37
pi :
alt_names
DDB1
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF
geneDDB1
id1642
genbank_ids
NM_001923
mutation
nameDDB1 (Damage-specific DNA binding protein 1)
shRNA_sequence
size3420
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
resistance markers :
593
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA