This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDS170-enNTS1
catalog :
199152
citations: 1
Reference
Bumbak F, Pons M, Inoue A, Paniagua J, Yan F, Wu H, et al. Ligands selectively tune the local and global motions of neurotensin receptor 1 (NTS1). Cell Rep. 2023;42:112015 pubmed publisher
product information
Catalog Number :
199152
Product Name :
pDS170-enNTS1
article :
doi10.1016/j.celrep.2023.112015
id28233980
pubmed_id36680775
bacterial resistance :
Ampicillin
cloning :
backbonepDS170
backbone_mutation
backbone_originInternal Lab Plasmid
backbone_size6112
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
For additional information, please refer to: Bumbak et al. 2018 Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies BBA Biomembranes. https://doi.org/10.1016/j.bbamem.2018.03.020 Bumbak et al. 2019 Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies Methods Mol Biol. https://doi.org/10.1007/978-1-4939-9121-1_3
growth strain :
JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)
origin :
37
pi :
alt_names
Thermostabilised rat neurotensin receptor 1
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5BamHI
promoterLac/T5
sequencing_primer_3ATCCATGCCATGTGTAATCCCAG
sequencing_primer_5CTGAAAGACGCGCAGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNtsr
geneNtsr1
id366274
genbank_ids
mutationV208M, C273S, C391S and C393S (over NTS1-B5)
nameenNTS1
shRNA_sequence
size1122
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagMBP
locationC terminal on backbone
tagmuGFP
locationN terminal on backbone
tag10xHis tag
locationC terminal on backbone
tagAvi tag
resistance markers :
5352
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA