This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-HA2-DDB1
catalog :
19909
citations: 4
Reference
Zhang H, Chen G, Feng X, Song H, Meng L, Fu Y, et al. Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progression. EMBO Mol Med. 2023;15:e15924 pubmed publisher
Nowak M, Suenkel B, Porras P, Migotti R, Schmidt F, Kny M, et al. DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy. J Cell Sci. 2019;132: pubmed publisher
Wang Y, Chen J, Chang C, Jen J, Huang T, Chen C, et al. Ubiquitination of tumor suppressor PML regulates prometastatic and immunosuppressive tumor microenvironment. J Clin Invest. 2017;127:2982-2997 pubmed publisher
Hu J, Zacharek S, He Y, Lee H, Shumway S, Duronio R, et al. WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. Genes Dev. 2008;22:866-71 pubmed publisher
product information
Catalog Number :
19909
Product Name :
pcDNA3-HA2-DDB1
article :
doi10.1101/gad.1624008
id2346
pubmed_id18381890
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-HA2
backbone_mutation
backbone_origin
backbone_size5512
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that Addgene's quality control result found mutations I253T, I258L and M679V in DDB1 when compared to the Genbank NM_001923. The plasmid should function as described in the original publication (Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71).
origin :
37
pi :
alt_names
DDB1
cloning
clone_methodRestriction Enzyme
cloning_site_3--
cloning_site_5--
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF
geneDDB1
id1642
genbank_ids
NM_001923
mutationplease see depositor comment below
nameDamage-specific DNA binding protein 1
shRNA_sequence
size3420
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
resistance markers :
593
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA