This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-myc3-CUL1
catalog :
19896
citations: 13
Reference
Lee D, Yoon E, Ham S, Lee K, Jang H, Woo D, et al. Diabetic sensory neuropathy and insulin resistance are induced by loss of UCHL1 in Drosophila. Nat Commun. 2024;15:468 pubmed publisher
H xe4 nle Kreidler S, Richter K, Hoffmann I. The SCF-FBXW7 E3 ubiquitin ligase triggers degradation of histone 3 lysine 4 methyltransferase complex component WDR5 to prevent mitotic slippage. J Biol Chem. 2022;298:102703 pubmed publisher
Wang Q, Li H, Tajima K, Verkerke A, Taxin Z, Hou Z, et al. Post-translational control of beige fat biogenesis by PRDM16 stabilization. Nature. 2022;609:151-158 pubmed publisher
Yin C, Lui E, Jiang T, Qi R. Proteolysis of γ-tubulin small complex proteins is mediated by the ubiquitin-proteasome system. FEBS Lett. 2021;595:1987-1996 pubmed publisher
Naik N, Lee S, Alonso J, Toth Z. KDM2B Overexpression Facilitates Lytic De Novo KSHV Infection by Inducing AP-1 Activity Through Interaction with the SCF E3 Ubiquitin Ligase Complex. J Virol. 2021;: pubmed publisher
Bayraktar G, Yuanxiang P, Confettura A, Gomes G, Raza S, Stork O, et al. Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus. Neuropsychopharmacology. 2020;45:2120-2130 pubmed publisher
Lasso G, Mayer S, Winkelmann E, Chu T, Elliot O, Patiño Galindo J, et al. A Structure-Informed Atlas of Human-Virus Interactions. Cell. 2019;178:1526-1541.e16 pubmed publisher
Zhou W, Xu J, Tan M, Li H, Li H, Wei W, et al. UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F. Mol Cell. 2018;70:1008-1024.e6 pubmed publisher
Mudhasani R, Tran J, Retterer C, Kota K, Whitehouse C, Bavari S. Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase. PLoS Pathog. 2016;12:e1005437 pubmed publisher
González Terán B, López J, Rodríguez E, Leiva L, Martínez Martínez S, Bernal J, et al. p38γ and δ promote heart hypertrophy by targeting the mTOR-inhibitory protein DEPTOR for degradation. Nat Commun. 2016;7:10477 pubmed publisher
Pentecost M, Vashisht A, Lester T, Voros T, Beaty S, Park A, et al. Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins. PLoS Pathog. 2015;11:e1004739 pubmed publisher
Bhutani S, Das A, Maheshwari M, Lakhotia S, Jana N. Dysregulation of core components of SCF complex in poly-glutamine disorders. Cell Death Dis. 2012;3:e428 pubmed publisher
Ohta T, Michel J, Schottelius A, Xiong Y. ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol Cell. 1999;3:535-41 pubmed
product information
Catalog Number :
19896
Product Name :
pcDNA3-myc3-CUL1
article :
doi10.1016/S1097-2765(00)80482-7
id2345
pubmed_id10230407
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5437
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
When we made this plasmid, we put a Nco1 site at the start codon (original cDNA was CAATGTCG but they mutated to CcATGgCG). It made an S2A mutation. There is also a K689E mutation. These mutations have no effect on protein function.
origin :
37
pi :
alt_names
CUL1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NcoI
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCUL1
id8454
genbank_ids
NP_003583
mutation
namecullin 1
shRNA_sequence
size2260
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmyc3
resistance markers :
593
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA