This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFRT/TO/FLAG/HA-DEST DICER
catalog :
19881
citations: 14
Reference
Liao G, Tseng Y, Tseng C, Lo C, Hsu W. The orf virus (ORFV) protein OV20.0 interacts with the microprocessor complex subunit DGCR8 to regulate miRNA biogenesis and ORFV infection. FEBS Lett. 2021;595:2897-2908 pubmed publisher
Lemus Diaz N, Ferreira R, Bohnsack K, Gruber J, Bohnsack M. The human box C/D snoRNA U3 is a miRNA source and miR-U3 regulates expression of sortin nexin 27. Nucleic Acids Res. 2020;: pubmed publisher
Vedanayagam J, Chatila W, Aksoy B, Majumdar S, Skanderup A, Demir E, et al. Cancer-associated mutations in DICER1 RNase IIIa and IIIb domains exert similar effects on miRNA biogenesis. Nat Commun. 2019;10:3682 pubmed publisher
Bouvette J, Korkut D, Fouillen A, Amellah S, Nanci A, Durocher Y, et al. High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension. BMC Biotechnol. 2018;18:76 pubmed publisher
Wu X, Yang Y, Huang Y, Chen Y, Wang T, Wu S, et al. RNA-binding protein AUF1 suppresses miR-122 biogenesis by down-regulating Dicer1 in hepatocellular carcinoma. Oncotarget. 2018;9:14815-14827 pubmed publisher
Antoniou A, Khudayberdiev S, Idziak A, Bicker S, Jacob R, Schratt G. The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development. EMBO Rep. 2018;19: pubmed publisher
Chitale S, Richly H. DICER- and MMSET-catalyzed H4K20me2 recruits the nucleotide excision repair factor XPA to DNA damage sites. J Cell Biol. 2018;217:527-540 pubmed publisher
Penha R, Sepe R, De Martino M, Esposito F, Pellecchia S, Raia M, et al. Role of Dicer1 in thyroid cell proliferation and differentiation. Cell Cycle. 2017;16:2282-2289 pubmed publisher
Lin S, Ptasinska A, Chen X, Shrestha M, Assi S, Chin P, et al. A FOXO1-induced oncogenic network defines the AML1-ETO preleukemic program. Blood. 2017;130:1213-1222 pubmed publisher
Chitale S, Richly H. DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair. Nucleic Acids Res. 2017;45:5901-5912 pubmed publisher
Harwig A, Jongejan A, van Kampen A, Berkhout B, Das A. Tat-dependent production of an HIV-1 TAR-encoded miRNA-like small RNA. Nucleic Acids Res. 2016;44:4340-53 pubmed publisher
Ren W, Shen S, Sun Z, Shu P, Shen X, Bu C, et al. Jak-STAT3 pathway triggers DICER1 for proteasomal degradation by ubiquitin ligase complex of CUL4A(DCAF1) to promote colon cancer development. Cancer Lett. 2016;375:209-220 pubmed publisher
Martinez N, Gregory R. Argonaute2 expression is post-transcriptionally coupled to microRNA abundance. RNA. 2013;19:605-12 pubmed publisher
Landthaler M, Gaidatzis D, Rothballer A, Chen P, Soll S, Dinic L, et al. Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. RNA. 2008;14:2580-96 pubmed publisher
product information
Catalog Number :
19881
Product Name :
pFRT/TO/FLAG/HA-DEST DICER
article :
doi10.1261/rna.1351608
id2069
pubmed_id18978028
bacterial resistance :
Ampicillin
cloning :
backbonepFRT/TO/FLAG/HA-DEST
backbone_mutation
backbone_origin
backbone_size5421
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The Dicer insert contains an N1659Y mutation.
origin :
37
pi :
alt_names
DCR1; Dicer; HERNA; KIAA0928
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD
geneDICER1
id23405
genbank_ids
NM_177438
mutation
nameDicer 1, ribonuclease type III
shRNA_sequence
size5772
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG/HA
resistance markers :
536
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA