This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRevTRE MKL1-N100 S449A/T450A/S454A
catalog :
19849
citations: 1
Reference
Muehlich S, Wang R, Lee S, Lewis T, Dai C, Prywes R. Serum-induced phosphorylation of the serum response factor coactivator MKL1 by the extracellular signal-regulated kinase 1/2 pathway inhibits its nuclear localization. Mol Cell Biol. 2008;28:6302-13 pubmed publisher
product information
Catalog Number :
19849
Product Name :
pRevTRE MKL1-N100 S449A/T450A/S454A
article :
doi10.1128/MCB.00427-08
id2272
pubmed_id18694962
bacterial resistance :
Ampicillin
cloning :
backbonepRev TRE
backbone_mutation
backbone_originClontech
backbone_size6500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth notes :
The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.
origin :
37
pi :
alt_names
MAL, BSAC, MRTF-A
cloning
clone_methodRestriction Enzyme
cloning_site_3Sal I
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBSAC, MAL, MKL, MKL1, MRTF-A
geneMRTFA
id57591
genbank_ids
mutationdeleted amino acids 1-100 and changed Serine 449, Threonine 450 and Serine 454 to Alanines.
nameMKL1-N100 S449A/T450A/S454A
shRNA_sequence
size2800
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xFLAG
resistance markers :
399
tags :
Low Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA