pCin4-3xFLAG-MKL1 | Addgene 19838 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCin4-3xFLAG-MKL1

catalog :

19838

citations: 2

Reference
Le Dour C, Chatzifrangkeskou M, Macquart C, Magiera M, Peccate C, Jouve C, et al. Actin-microtubule cytoskeletal interplay mediated by MRTF-A/SRF signaling promotes dilated cardiomyopathy caused by LMNA mutations. Nat Commun. 2022;13:7886 pubmed publisher
Muehlich S, Wang R, Lee S, Lewis T, Dai C, Prywes R. Serum-induced phosphorylation of the serum response factor coactivator MKL1 by the extracellular signal-regulated kinase 1/2 pathway inhibits its nuclear localization. Mol Cell Biol. 2008;28:6302-13 pubmed publisher

product information

Catalog Number :

19838

Product Name :

pCin4-3xFLAG-MKL1

article :

doi	10.1128/MCB.00427-08
id	2272
pubmed_id	18694962

bacterial resistance :

Ampicillin

cloning :

backbone

pcin4

backbone_mutation

backbone_origin

S Rees et al., BioTechniques 1996

backbone_size

5257

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.

origin :

pi :

alt_names

MAL, BSAC, MRTF-A

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	NA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BSAC, MAL, MKL, MKL1, MRTF-A
gene	MRTFA
id	57591

genbank_ids

mutation

name

MKL1

shRNA_sequence

size

2800

species

9606

Homo sapiens