This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCW57.1-pDUXChd:hDUX4ctd-ca
catalog :
198237
citations: 1
Reference
Nip Y, Bennett S, Smith A, Jones T, Jones P, Tapscott S. Human DUX4 and porcine DUXC activate similar early embryonic programs in pig muscle cells: implications for preclinical models of FSHD. Hum Mol Genet. 2023;32:1864-1874 pubmed publisher
product information
Catalog Number :
198237
Product Name :
pCW57.1-pDUXChd:hDUX4ctd-ca
article :
doi10.1093/hmg/ddad021
id28233889
pubmed_id36728804
bacterial resistance :
Ampicillin
cloning :
backbonepCW57.1
backbone_mutation
backbone_origin
backbone_size9354
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)
growth temp :
We used Stbl3 cells (recombination deficient)
origin :
37
pi :
alt_names
porcine DUXC/human DUX4 chimera (codon altered)
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NheI
promoterTRE
sequencing_primer_3
sequencing_primer_5aggtaggcgtgtacggtgg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDUX4L
geneDUX4
id100288687
genbank_ids
mutationcodon altered
nameDUX4L pDUXChd:hDUX4ctd (codon altered)
shRNA_sequence
size1320
species
9606
Homo sapiens
32644
Other
S. scrofa (porcine)
tags
resistance markers :
520
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA