This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pminiT-MED6-mEmerald donor
catalog :
197865
product information
Catalog Number :
197865
Product Name :
pminiT-MED6-mEmerald donor
article :
doi10.1101/2022.10.26.513917
id28233821
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepminiT
backbone_mutationadd Med6 homology arms around C terminal with a mEmerald coding sequence
backbone_originNEB
backbone_size2525
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mouse Targeting
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2022.10.26.513917v1 for bioRxiv preprint. Addgene QC found S228G mutation in mEmerald that does not affect plasmid function.
growth strain :
Donor plasmid used for building MED6-mEmerald knock-in mESCs
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNA
sequencing_primer_3CACGGTGCTGAGGTATAGC
sequencing_primer_5GATAAAGAGGAACGCAGAATG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases1500012F11Rik
geneMed6
id69792
genbank_ids
NM_001347384.1
NP_001334313.1
mutation
nameMed6
shRNA_sequence
size2200
species
10090
Mus musculus
tags
locationC terminal on backbone
tagmEmerald
resistance markers :
3392
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA