This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FU-tet-o-hOct4
catalog :
19778
citations: 14
Reference
Tao Y, Yang Y, Yang Z, Wang L, Wang S, Zhao Y. Robust small molecule-aided cardiac reprogramming systems selective to cardiac fibroblasts. iScience. 2023;26:108466 pubmed publisher
Maloshenok L, Abushinova G, Kazachkina N, Bogdanov A, Zherdeva V. Tet-Regulated Expression and Optical Clearing for In Vivo Visualization of Genetically Encoded Chimeric dCas9/Fluorescent Protein Probes. Materials (Basel). 2023;16: pubmed publisher
Li K, Sun X, Zha R, Liu S, Feng Y, Sano T, et al. Counterintuitive production of tumor-suppressive secretomes from Oct4- and c-Myc-overexpressing tumor cells and MSCs. Theranostics. 2022;12:3084-3103 pubmed publisher
Abdel Raouf K, Rezgui R, Stefanini C, Teo J, Christoforou N. Transdifferentiation of Human Fibroblasts into Skeletal Muscle Cells: Optimization and Assembly into Engineered Tissue Constructs through Biological Ligands. Biology (Basel). 2021;10: pubmed publisher
Zhao H, Zhang Y, Xu X, Sun Q, Yang C, Wang H, et al. Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury. Front Cell Dev Biol. 2021;9:608367 pubmed publisher
Li K, Ning T, Wang H, Jiang Y, Zhang J, Ge Z. Nanosecond pulsed electric fields enhance mesenchymal stem cells differentiation via DNMT1-regulated OCT4/NANOG gene expression. Stem Cell Res Ther. 2020;11:308 pubmed publisher
Zolotarenko A, Chekalin E, Piruzian E, Bruskin S. FRA1 mediates the activation of keratinocytes: Implications for the development of psoriatic plaques. Biochim Biophys Acta Mol Basis Dis. 2018;1864:3726-3734 pubmed publisher
Mayor Ruiz C, Dominguez O, Fernandez Capetillo O. TrapSeq: An RNA Sequencing-Based Pipeline for the Identification of Gene-Trap Insertions in Mammalian Cells. J Mol Biol. 2017;429:2780-2789 pubmed publisher
Boularaoui S, Abdel Raouf K, Alwahab N, Kondash M, Truskey G, Teo J, et al. Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle. J Tissue Eng Regen Med. 2018;12:e918-e936 pubmed publisher
Christoforou N, Chakraborty S, Kirkton R, Adler A, Addis R, Leong K. Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage. Sci Rep. 2017;7:40285 pubmed publisher
Ji H, Atchison L, Chen Z, Chakraborty S, Jung Y, Truskey G, et al. Transdifferentiation of human endothelial progenitors into smooth muscle cells. Biomaterials. 2016;85:180-194 pubmed publisher
Christoforou N, Chellappan M, Adler A, Kirkton R, Wu T, Addis R, et al. Transcription factors MYOCD, SRF, Mesp1 and SMARCD3 enhance the cardio-inducing effect of GATA4, TBX5, and MEF2C during direct cellular reprogramming. PLoS ONE. 2013;8:e63577 pubmed publisher
Addis R, Hsu F, Wright R, Dichter M, Coulter D, Gearhart J. Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS ONE. 2011;6:e28719 pubmed publisher
Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell. 2008;3:340-5 pubmed publisher
product information
Catalog Number :
19778
Product Name :
FU-tet-o-hOct4
article :
doi10.1016/j.stem.2008.08.003
id2333
pubmed_id18786420
bacterial resistance :
Ampicillin
cloning :
backboneFUdeltaGW
backbone_mutation
backbone_origin
backbone_size8400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
From article, concerning viral infections: For a standard infection in a 35 mmdish (aprox 10^5 cells), 10 microL rtTA+5 microL factors (OCT4, SOX2, KLF4, and NANOG)+2 microL cMYC was used in an overnight infection supplemented with 6 microg/microL polybrene.
growth temp :
Stbl3
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
mutation
nameOct4
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
resistance markers :
203
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA