This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FU-tet-o-hNanogP8
catalog :
19776
citations: 2
Reference
Lebedeva O, Poberezhniy D, Novosadova E, Gerasimova T, Novosadova L, Arsenyeva E, et al. Overexpression of Parkin in the Neuronal Progenitor Cells from a Patient with Parkinson's Disease Shifts the Transcriptome Towards the Normal State. Mol Neurobiol. 2023;60:3522-3533 pubmed publisher
Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell. 2008;3:340-5 pubmed publisher
product information
Catalog Number :
19776
Product Name :
FU-tet-o-hNanogP8
article :
doi10.1016/j.stem.2008.08.003
id2333
pubmed_id18786420
bacterial resistance :
Ampicillin
cloning :
backboneFUdeltaGW
backbone_mutation
backbone_origin
backbone_size8400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
From article, concerning viral infections: For a standard infection in a 35 mmdish (aprox 10^5 cells), 10 microL rtTA+5 microL factors (OCT4, SOX2, KLF4, and NANOG)+2 microL cMYC was used in an overnight infection supplemented with 6 microg/microL polybrene.
growth temp :
Stbl3
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNANOGP1, PN8
geneNANOGP8
id388112
genbank_ids
mutation
nameNanogP8
shRNA_sequence
size917
species
9606
Homo sapiens
tags
resistance markers :
203
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA