FU-tet-o-hc-myc | Addgene 19775 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

FU-tet-o-hc-myc

catalog :

19775

citations: 3

Reference
Afifi M, Crncec A, Cornwell J, Cataisson C, Paul D, Ghorab L, et al. Irreversible cell cycle exit associated with senescence is mediated by constitutive MYC degradation. Cell Rep. 2023;42:113079 pubmed publisher
Zheng X, Boyer L, Jin M, Mertens J, Kim Y, Ma L, et al. Metabolic reprogramming during neuronal differentiation from aerobic glycolysis to neuronal oxidative phosphorylation. elife. 2016;5: pubmed publisher
Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell. 2008;3:340-5 pubmed publisher

product information

Catalog Number :

19775

Product Name :

FU-tet-o-hc-myc

article :

doi	10.1016/j.stem.2008.08.003
id	2333
pubmed_id	18786420

bacterial resistance :

Ampicillin

cloning :

backbone

FUdeltaGW

backbone_mutation

backbone_origin

backbone_size

8400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

From article, concerning viral infections: For a standard infection in a 35 mmdish (aprox 10^5 cells), 10 microL rtTA+5 microL factors (OCT4, SOX2, KLF4, and NANOG)+2 microL cMYC was used in an overnight infection supplemented with 6 microg/microL polybrene.

growth temp :

Stbl3

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	WPRE-R
sequencing_primer_5	LNCX
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

T58A

name

c-myc

shRNA_sequence

size

1320

species

9606

Homo sapiens