pAd-cMyc T58A | Addgene 19769 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAd-cMyc T58A

catalog :

19769

citations: 1

Reference
Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Induced pluripotent stem cells generated without viral integration. Science. 2008;322:945-9 pubmed publisher

product information

Catalog Number :

19769

Product Name :

pAd-cMyc T58A

article :

doi	10.1126/science.1162494
id	2326
pubmed_id	18818365

bacterial resistance :

Ampicillin

cloning :

backbone

pAd-ClaI

backbone_mutation

backbone_origin

backbone_size

32900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Adenoviral

growth notes :

From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence was isolated from the resulting plasmids by MfeI/PacI digestion and used for electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones were identified by HindIII digestion.

growth temp :

Stbl3

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	PacI
cloning_site_5	MfeI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

T58A

name

c-myc

shRNA_sequence

size

3600

species

9606

Homo sapiens