This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLV-tetO-myc T58A
catalog :
19763
citations: 4
Reference
Chen H, Gardner E, Shah Y, Zhang K, Thakur A, Zhang C, et al. FORMATION OF MALIGNANT, METASTATIC SMALL CELL LUNG CANCERS THROUGH OVERPRODUCTION OF cMYC PROTEIN IN TP53 AND RB1 DEPLETED PULMONARY NEUROENDOCRINE CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS. bioRxiv. 2023;: pubmed publisher
Afifi M, Crncec A, Cornwell J, Cataisson C, Paul D, Ghorab L, et al. Irreversible cell cycle exit associated with senescence is mediated by constitutive MYC degradation. Cell Rep. 2023;42:113079 pubmed publisher
Kharbanda A, Walter D, Gudiel A, Schek N, Feldser D, Witze E. Blocking EGFR palmitoylation suppresses PI3K signaling and mutant KRAS lung tumorigenesis. Sci Signal. 2020;13: pubmed publisher
Stadtfeld M, Maherali N, Breault D, Hochedlinger K. Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell. 2008;2:230-40 pubmed publisher
product information
Catalog Number :
19763
Product Name :
pLV-tetO-myc T58A
article :
doi10.1016/j.stem.2008.02.001
id2327
pubmed_id18371448
bacterial resistance :
Ampicillin
cloning :
backboneFUdeltaGW
backbone_mutation
backbone_origin
backbone_size9941
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
growth temp :
Stbl3
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutationT58A
namec-myc
shRNA_sequence
size1320
species
9606
Homo sapiens
tags
locationN terminal on insert
tag3xHA
resistance markers :
203
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA