pLKO.1.sh.beta-catenin.2279 | Addgene 19762 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLKO.1.sh.beta-catenin.2279

catalog :

19762

citations: 8

Reference
Cheng F, Wang X, Chiou Y, He C, Guo H, Tan Y, et al. Trefoil factor 3 promotes pancreatic carcinoma progression via WNT pathway activation mediated by enhanced WNT ligand expression. Cell Death Dis. 2022;13:265 pubmed publisher
Zhou X, Geng L, Wang D, Yi H, Talmon G, Wang J. R-Spondin1/LGR5 Activates TGFβ Signaling and Suppresses Colon Cancer Metastasis. Cancer Res. 2017;77:6589-6602 pubmed publisher
Li L, Sheng Y, Li W, Hu C, Mittal N, Tohyama K, et al. β-Catenin Is a Candidate Therapeutic Target for Myeloid Neoplasms with del(5q). Cancer Res. 2017;77:4116-4126 pubmed publisher
Huang T, Alvarez A, Pangeni R, Horbinski C, Lu S, Kim S, et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun. 2016;7:12885 pubmed publisher
Koziński K, Jazurek M, Dobrzyn P, Janikiewicz J, Kolczyńska K, Gajda A, et al. Adipose- and muscle-derived Wnts trigger pancreatic ?-cell adaptation to systemic insulin resistance. Sci Rep. 2016;6:31553 pubmed publisher
Qu Y, Gharbi N, Yuan X, Olsen J, Blicher P, Dalhus B, et al. Axitinib blocks Wnt/β-catenin signaling and directs asymmetric cell division in cancer. Proc Natl Acad Sci U S A. 2016;113:9339-44 pubmed publisher
Pai P, Rachagani S, Lakshmanan I, Macha M, Sheinin Y, Smith L, et al. The canonical Wnt pathway regulates the metastasis-promoting mucin MUC4 in pancreatic ductal adenocarcinoma. Mol Oncol. 2016;10:224-39 pubmed publisher
Firestein R, Bass A, Kim S, Dunn I, Silver S, Guney I, et al. CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature. 2008;455:547-51 pubmed publisher

product information

Catalog Number :

19762

Product Name :

pLKO.1.sh.beta-catenin.2279

article :

doi	10.1038/nature07179
id	2325
pubmed_id	18794900

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1 puro

backbone_mutation

backbone_origin

backbone_size

7032

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

CCGGGCTTGGAATGAGACTGCTGATCTCGAGATCAGCAG
TCTCATTCCAAGCTTTTT
2279 refers to the shRNA clone name from the RNAi Consortium at the Broad Institute and indicates the location on the CTNNB1 mRNA that is targeted. shRNA sequence for 2279 is:

origin :

pi :

alt_names

CTNNB

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	LKO.1 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CTNNB, EVR7, MRD19, NEDSDV, armadillo
gene	CTNNB1
id	1499

genbank_ids

mutation

name

small hairpin RNA against beta-catenin

shRNA_sequence

size

species

9606

Homo sapiens