This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO.1.sh.beta-catenin.1248
catalog :
19761
citations: 13
Reference
Cheng F, Wang X, Chiou Y, He C, Guo H, Tan Y, et al. Trefoil factor 3 promotes pancreatic carcinoma progression via WNT pathway activation mediated by enhanced WNT ligand expression. Cell Death Dis. 2022;13:265 pubmed publisher
Sayedyahossein S, Huang K, Li Z, Zhang C, Kozlov A, Johnston D, et al. Pannexin 1 binds β-catenin to modulate melanoma cell growth and metabolism. J Biol Chem. 2021;296:100478 pubmed publisher
Jiang Y, Han Q, Zhao H, Zhang J. Promotion of epithelial-mesenchymal transformation by hepatocellular carcinoma-educated macrophages through Wnt2b/β-catenin/c-Myc signaling and reprogramming glycolysis. J Exp Clin Cancer Res. 2021;40:13 pubmed publisher
You C, Zhang S, Sun Y, Zhang S, Tang G, Tang F, et al. β-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors. Int J Oncol. 2018;53:2258-2268 pubmed publisher
Kim J, Lee J, Hwang H, Lee D, Park C, Jung S, et al. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells. Exp Mol Med. 2018;50:24 pubmed publisher
Zhou X, Geng L, Wang D, Yi H, Talmon G, Wang J. R-Spondin1/LGR5 Activates TGFβ Signaling and Suppresses Colon Cancer Metastasis. Cancer Res. 2017;77:6589-6602 pubmed publisher
Li L, Sheng Y, Li W, Hu C, Mittal N, Tohyama K, et al. β-Catenin Is a Candidate Therapeutic Target for Myeloid Neoplasms with del(5q). Cancer Res. 2017;77:4116-4126 pubmed publisher
Huang T, Alvarez A, Pangeni R, Horbinski C, Lu S, Kim S, et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun. 2016;7:12885 pubmed publisher
Pai P, Rachagani S, Dhawan P, Sheinin Y, Macha M, Qazi A, et al. MUC4 is negatively regulated through the Wnt/?-catenin pathway via the Notch effector Hath1 in colorectal cancer. Genes Cancer. 2016;7:154-168 pubmed publisher
Qu Y, Gharbi N, Yuan X, Olsen J, Blicher P, Dalhus B, et al. Axitinib blocks Wnt/β-catenin signaling and directs asymmetric cell division in cancer. Proc Natl Acad Sci U S A. 2016;113:9339-44 pubmed publisher
Pai P, Rachagani S, Lakshmanan I, Macha M, Sheinin Y, Smith L, et al. The canonical Wnt pathway regulates the metastasis-promoting mucin MUC4 in pancreatic ductal adenocarcinoma. Mol Oncol. 2016;10:224-39 pubmed publisher
Cheng Y, Cheung A, Ko J, Phoon Y, Chiu P, Lo P, et al. Physiological β-catenin signaling controls self-renewal networks and generation of stem-like cells from nasopharyngeal carcinoma. BMC Cell Biol. 2013;14:44 pubmed publisher
Firestein R, Bass A, Kim S, Dunn I, Silver S, Guney I, et al. CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature. 2008;455:547-51 pubmed publisher
product information
Catalog Number :
19761
Product Name :
pLKO.1.sh.beta-catenin.1248
article :
doi10.1038/nature07179
id2325
pubmed_id18794900
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1 puro
backbone_mutation
backbone_origin
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
CCGGAGGTGCTATCTGTCTGCTCTACTCGAGTAGAGCAG
ACAGATAGCACCTTTTTT
1248 refers to the shRNA clone name from the RNAi Consortium at the Broad Institute and indicates the location on the CTNNB1 mRNA that is targeted. shRNA sequence for 1248 is:
origin :
37
pi :
alt_names
CTNNB
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCTNNB, EVR7, MRD19, NEDSDV, armadillo
geneCTNNB1
id1499
genbank_ids
mutation
namesmall hairpin RNA against beta-catenin
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
184
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA