This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Orai1 YFP
catalog :
19756
citations: 18
Reference
Kodakandla G, West S, Wang Q, Tewari R, Zhu M, Akimzhanov A, et al. Dynamic S-acylation of the ER-resident protein stromal interaction molecule 1 (STIM1) is required for store-operated Ca2+ entry. J Biol Chem. 2022;298:102303 pubmed publisher
Wang W, Demaurex N. The mammalian trafficking chaperone protein UNC93B1 maintains the ER calcium sensor STIM1 in a dimeric state primed for translocation to the ER cortex. J Biol Chem. 2022;298:101607 pubmed publisher
Carreras Sureda A, Abrami L, Ji Hee K, Wang W, Henry C, Frieden M, et al. S-acylation by ZDHHC20 targets ORAI1 channels to lipid rafts for efficient Ca2+ signaling by Jurkat T cell receptors at the immune synapse. elife. 2021;10: pubmed publisher
Cheng P, Tian X, Tang W, Cheng J, Bao J, Wang H, et al. Direct control of store-operated calcium channels by ultrafast laser. Cell Res. 2021;: pubmed publisher
Singh A, Van Slyke A, Sirenko M, Song A, Kammermeier P, Zipfel W. Stoichiometric analysis of protein complexes by cell fusion and single molecule imaging. Sci Rep. 2020;10:14866 pubmed publisher
Rowell T, Keating J, Zorn B, Glish G, Shears S, Tarran R. Flavored e-liquids increase cytoplasmic Ca2+ levels in airway epithelia. Am J Physiol Lung Cell Mol Physiol. 2020;318:L226-L241 pubmed publisher
Diercks B, Werner R, Weidemüller P, Czarniak F, Hernandez L, Lehmann C, et al. ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation. Sci Signal. 2018;11: pubmed publisher
Bulla M, Gyimesi G, Kim J, Bhardwaj R, Hediger M, Frieden M, et al. ORAI1 channel gating and selectivity is differentially altered by natural mutations in the first or third transmembrane domain. J Physiol. 2019;597:561-582 pubmed publisher
Zheng S, Leclerc G, Li B, Swords R, Barredo J. Inhibition of the NEDD8 conjugation pathway induces calcium-dependent compensatory activation of the pro-survival MEK/ERK pathway in acute lymphoblastic leukemia. Oncotarget. 2018;9:5529-5544 pubmed publisher
Shen Y, Chen Y, Wu J, Shaner N, Campbell R. Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity. PLoS ONE. 2017;12:e0171257 pubmed publisher
Wu T, Huang J, Moore P, Little M, Walton W, Fellner R, et al. Identification of BPIFA1/SPLUNC1 as an epithelium-derived smooth muscle relaxing factor. Nat Commun. 2017;8:14118 pubmed publisher
Cai Z, Zhang A, Choksi S, Li W, Li T, Zhang X, et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res. 2016;26:886-900 pubmed publisher
Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, et al. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun. 2015;6:8021 pubmed publisher
Jozsef L, Tashiro K, Kuo A, Park E, Skoura A, Albinsson S, et al. Reticulon 4 is necessary for endoplasmic reticulum tubulation, STIM1-Orai1 coupling, and store-operated calcium entry. J Biol Chem. 2014;289:9380-95 pubmed publisher
Sheridan J, Gilmore R, Watson M, Archer C, Tarran R. 17?-Estradiol inhibits phosphorylation of stromal interaction molecule 1 (STIM1) protein: implication for store-operated calcium entry and chronic lung diseases. J Biol Chem. 2013;288:33509-18 pubmed publisher
Varadarajan S, Tanaka K, Smalley J, Bampton E, Pellecchia M, Dinsdale D, et al. Endoplasmic reticulum membrane reorganization is regulated by ionic homeostasis. PLoS ONE. 2013;8:e56603 pubmed publisher
Pham E, Mills E, Truong K. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol. 2011;18:880-90 pubmed publisher
Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan P. Orai1 is an essential pore subunit of the CRAC channel. Nature. 2006;443:230-3 pubmed
product information
Catalog Number :
19756
Product Name :
Orai1 YFP
article :
doi10.1038/nature05122
id2324
pubmed_id16921383
bacterial resistance :
Kanamycin
cloning :
backboneEGFP-N1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
ORAI calcium release-activated calcium modulator 1
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3EGFP-N
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCRACM1, IMD9, ORAT1, TAM2, TMEM142A
geneORAI1
id84876
genbank_ids
mutation
nameOrai1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagYFP
resistance markers :
410
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA