This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
B52_puro_gRNA_3418711_KCNB2
catalog :
197558
product information
Catalog Number :
197558
Product Name :
B52_puro_gRNA_3418711_KCNB2
article :
doi
id28233788
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneCMV+Puro_B52
backbone_mutationaddition of gRNA to region 3418711 upstream of KCNB2
backbone_originBagli Lab
backbone_size4044
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
gRNA with puromycin selection
growth notes :
Any publications using this clone should state: we thank the laboratory of Darius Bagli for the use of this clone . In addition, we have a forthcoming article which can be referenced once it is published.
growth strain :
gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)
origin :
37
pi :
alt_names
upstream Kv2.2 promoter gRNA at chr5:3418711 site
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoterU6
sequencing_primer_3waiting to find
sequencing_primer_5waiting to find
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKv2.2
geneKcnb2
id117105
genbank_ids
NM_054000
mutation
nameupstream KCNB2 promoter gRNA
shRNA_sequence
size734
species
10116
Rattus norvegicus
tags
location
tagpuromycin resistance cassette under CMV promotion
plasmid copy :
We had help from Genscript with the cloning, though the design was ours.
resistance markers :
6386
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA