This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-GST-FBW7 alpha
catalog :
197456
citations: 1
Reference
Khan O, Almagro J, Nelson J, Horswell S, Encheva V, Keyan K, et al. Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12. Nat Commun. 2021;12:2043 pubmed publisher
product information
Catalog Number :
197456
Product Name :
pDEST-GST-FBW7 alpha
article :
doi10.1038/s41467-021-22319-5
id28222797
pubmed_id33824312
bacterial resistance :
Ampicillin
cloning :
backbonepDEST-565
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
In vitro translation
growth notes :
Please note, plasmid contains an A871T mutation in FBW7. This mutation is outside of the WD40 propeller and is not expected to interfere with the function of the protein.
growth strain :
The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.
growth temp :
Used mainly for in vitro translation.
origin :
37
pi :
alt_names
hCDC4, FBW7 isoform 1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3
sequencing_primer_5T7 Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
geneFBXW7
id55294
genbank_ids
NM_001349798.2
mutationSee depositor comments below
nameFBW7
shRNA_sequence
size2000
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
6462
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA