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product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-Myc-FBW7 Delta D-Domain
catalog :
197453
citations: 1
Reference
Khan O, Almagro J, Nelson J, Horswell S, Encheva V, Keyan K, et al. Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12. Nat Commun. 2021;12:2043 pubmed publisher
product information
Catalog Number :
197453
Product Name :
pDEST-Myc-FBW7 Delta D-Domain
article :
doi10.1038/s41467-021-22319-5
id28222797
pubmed_id33824312
bacterial resistance :
Ampicillin
cloning :
backbonepDEST-Myc
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Gateway adapters were added to FBW7 dimerization deleted mutant and were cloned in to pDEST-N-myc. The insert is verified by western blotting.
growth strain :
The plasmid expresses D-domain deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression and detection by western blotting.
origin :
37
pi :
alt_names
hCDC4, FBW7 isoform 1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
geneFBXW7
id55294
genbank_ids
NM_001349798.2
mutationFBW& dimerization domain deleted.
nameFBW7
shRNA_sequence
size2000
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyc
plasmid copy :
The insert is PCR amplified from plasmid containing FBW7 dimerization deleted mutant which was from Welcker M et al Genes & Dev 2013.
resistance markers :
6462
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA