MSCV FLIP FF | Addgene 19744 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MSCV FLIP FF

catalog :

19744

citations: 1

Reference
Stern P, Astrof S, Erkeland S, Schustak J, Sharp P, Hynes R. A system for Cre-regulated RNA interference in vivo. Proc Natl Acad Sci U S A. 2008;105:13895-900 pubmed publisher

product information

Catalog Number :

19744

Product Name :

MSCV FLIP FF

article :

doi	10.1073/pnas.0806907105
id	2323
pubmed_id	18779577

bacterial resistance :

Ampicillin

cloning :

backbone

MSCV FLIP

backbone_mutation

backbone_origin

backbone_size

7926

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

RNAi

Cre/Lox

growth notes :

Constitutively expresses puromycin-resistance and the surface molecule Thy1.1. Recombines to express GFP and miR30-based RNAi. There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence. This is the control vector, which can be used for cloning. This vector expresses Firefly luciferase. See FLIP vector protocols (PDF from this page) for more information.

growth temp :

grow in stbl3 cells at 30oC

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	PmeI
cloning_site_5	NotI
promoter
sequencing_primer_3
sequencing_primer_5	GFP miR primer (GCTGGAGTTCGTGACCGCC)
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

oligo targeting Firefly luciferase

shRNA_sequence

size

386

species