pFsynW SYT1-PGM reporter | Addgene 197278 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFsynW SYT1-PGM reporter

catalog :

197278

citations: 1

Reference
Watson E, Pauers M, Seibert M, Vevea J, Chapman E. Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons. elife. 2023;12: pubmed publisher

product information

Catalog Number :

197278

Product Name :

pFsynW SYT1-PGM reporter

article :

doi	10.7554/eLife.82568
id	28233761
pubmed_id	36729040

bacterial resistance :

Ampicillin

cloning :

backbone

FUGW (#14883)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/2022.02.08.479521v1 for bioRxiv preprint.

growth strain :

Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	hSyn (human synapsin I promoter)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	P65
gene	Syt1
id	25716

genbank_ids

mutation

T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

name

SYT1 and HaloTag

shRNA_sequence

size

species

10116

Rattus norvegicus