This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
iYFP-Parkin Plasmid
catalog :
197107
citations: 1
Reference
Capelluto F, Alberico H, Ledo Hopgood P, Tilly J, Woods D. Lineage-Mismatched Mitochondrial Replacement in an Inducible Mitochondrial Depletion Model Effectively Restores the Original Proteomic Landscape of Recipient Cells. Adv Biol (Weinh). 2023;7:e2200246 pubmed publisher
product information
Catalog Number :
197107
Product Name :
iYFP-Parkin Plasmid
article :
doi10.1002/adbi.202200246
id28233748
pubmed_id36651121
bacterial resistance :
Ampicillin
cloning :
backbonepRetroX-TetOne Puro
backbone_mutation
backbone_originTakeda
backbone_size10000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Retroviral
growth notes :
Please note: Plasmid contains an I142V mutation in Parkinson protein 2, E3 ubiquitin protein ligase compared to the NCBI reference sequence NP_004553.2. This mutation is not expected to affect plasmid function.
growth strain :
Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.
origin :
37
pi :
alt_names
Parkin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_35-ATGGATCCTTACACGTCGAAC-3
sequencing_primer_55-TTGAATTCATGGTGAGCAAGG-3
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesAR-JP, LPRS2, PARK2, PDJ
genePRKN
id5071
genbank_ids
NM_004562.3
mutation
nameparkinson protein 2, E3 ubiquitin protein ligase
shRNA_sequence
size1400
species
9606
Homo sapiens
tags
locationN terminal on insert
tagYFP
plasmid copy :
We utilized the YFP-Parkin insert from Dr. Stephen Tait's original plasmid (Plasmid #61728).
resistance markers :
6412
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA