This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGAD424-Rabex-5
catalog :
197043
citations: 1
Reference
Mattera R, Tsai Y, Weissman A, Bonifacino J. The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain. J Biol Chem. 2006;281:6874-83 pubmed
product information
Catalog Number :
197043
Product Name :
pGAD424-Rabex-5
article :
doi10.1074/jbc.M509939200
id28233709
pubmed_id16407276
bacterial resistance :
Ampicillin
cloning :
backbonepGAD424
backbone_mutation
backbone_origin
backbone_size6665
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
NotI/SpeI fragment encoding Rabex-5 was polished wIth T4 DNA polymerse and subcloned into pGAD424 digested with BamH1 and also polished wIth T4 DNA polymerase. Insert contains an additional N-terminal GRSSP sequence preceding the Rabex-5 initiation codon.
growth strain :
Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5BamH1
promoterADH1
sequencing_primer_3GAL4 transcriptional activation domain reverse
sequencing_primer_5GAL4 transcriptional activation domain forward
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesRABEX5
geneRABGEF1
id282335
genbank_ids
mutation
nameRabex-5
shRNA_sequence
size1527
species
9913
Bos taurus
tags
locationN terminal on backbone
tagGAL4 transcriptional activation domain (AD) fragment
resistance markers :
1784
tags :
Unknown
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA