pET28a_DmKHC[1-421]-SNAP-6xHis | Addgene 196975 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET28a_DmKHC[1-421]-SNAP-6xHis

catalog :

196975

citations: 1

Reference
Deguchi T, Iwanski M, Schentarra E, Heidebrecht C, Schmidt L, Heck J, et al. Direct observation of motor protein stepping in living cells using MINFLUX. Science. 2023;379:1010-1015 pubmed publisher

product information

Catalog Number :

196975

Product Name :

pET28a_DmKHC[1-421]-SNAP-6xHis

article :

doi	10.1126/science.ade2676
id	28243375
pubmed_id	36893247

bacterial resistance :

Kanamycin

cloning :

backbone

pET28a

backbone_mutation

backbone_origin

backbone_size

5212

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

growth temp :

Purification from BL21. Induce protein expression with IPTG. After induction, grow at 18 degrees C.

origin :

pi :

2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgsgeneKhcid36810genbank_idsmutationnameKinesin Heavy ChainshRNA_sequencesize1839species

7227

Drosophila melanogaster