This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen
catalog :
196974
citations: 1
Reference
Chen F, Wu J, Iwanski M, Jurriens D, Sandron A, Pasolli M, et al. Self-assembly of pericentriolar material in interphase cells lacking centrioles. elife. 2022;11: pubmed publisher
product information
Catalog Number :
196974
Product Name :
pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen
article :
doi10.7554/eLife.77892
id28243374
pubmed_id35787744
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6p-1
backbone_mutation
backbone_origin
backbone_size4944
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag
growth temp :
Purification from BL21. Induce protein expression with IPTG. After induction, grow at 18 degrees C.
origin :
37
pi :
alt_names
KHC, Khc, kinesin-1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotertac
sequencing_primer_3GGAGCTGCATGTGTCAGAGG
sequencing_primer_5GGCAAGCCACGTTTGGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG7765, 2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgs
geneKhc
id36810
genbank_ids
mutation
nameKinesin Heavy Chain
shRNA_sequence
size1980
species
7227
Drosophila melanogaster
tags
locationN terminal on insert
tagGST
locationC terminal on insert
tagmNeonGreen
resistance markers :
3868
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA