This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBactin-PACT-mOrange2-hCM1
catalog :
196868
citations: 1
Reference
Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, et al. Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Neuron. 2023;111:1241-1263.e16 pubmed publisher
product information
Catalog Number :
196868
Product Name :
pBactin-PACT-mOrange2-hCM1
article :
doi10.1016/j.neuron.2023.01.020
id28233695
pubmed_id36796357
bacterial resistance :
Ampicillin
cloning :
backbonepBeta-actin-Nedd1-mOrange2-hCM1
backbone_mutation
backbone_origin
backbone_size6972
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NheI
promoterChicken bactin (plus Chicken bactin intron)
sequencing_primer_3 SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCG-NAP, Gisp, Yotiao
geneAkap9
id246150
genbank_ids
mutation
namePACT-mOrange2-hCM1
shRNA_sequence
size815
species
10116
Rattus norvegicus
tags
plasmid copy :
pBeta-actin [Kapitein et al. , 2010]
resistance markers :
4675
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA