pCR-XL-rAkap9 | Addgene 196867 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCR-XL-rAkap9

catalog :

196867

citations: 1

Reference
Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, et al. Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Neuron. 2023;111:1241-1263.e16 pubmed publisher

product information

Catalog Number :

196867

Product Name :

pCR-XL-rAkap9

article :

doi	10.1016/j.neuron.2023.01.020
id	28233695
pubmed_id	36796357

bacterial resistance :

Kanamycin

cloning :

backbone

pCR-XL-TOPO

backbone_mutation

backbone_origin

backbone_size

3519

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

For successful transformation of Plasmid 196867: pCR-XL-rAkap9, it is recommended to use electroporation and to grow transformed bacteria at no more than 28 C for two days and with 30 g/ml kanamycin on LB plates for full length plasmid production

growth strain :

Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

growth temp :

Grow 2-3 days at room temperature. See comments section for more details.

origin :

pi :

alt_names

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter	Lac promoter and T7 promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CG-NAP, Gisp, Yotiao
gene	Akap9
id	246150

genbank_ids

mutation

name

Akap9

shRNA_sequence

size

11505

species

10116

Rattus norvegicus