This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBactin-Nedd1-mOrange2-F75A
catalog :
196862
citations: 1
Reference
Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, et al. Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Neuron. 2023;111:1241-1263.e16 pubmed publisher
product information
Catalog Number :
196862
Product Name :
pBactin-Nedd1-mOrange2-F75A
article :
doi10.1016/j.neuron.2023.01.020
id28233695
pubmed_id36796357
bacterial resistance :
Ampicillin
cloning :
backbonepBeta-actin-Nedd1-mOrange2
backbone_mutation
backbone_origin
backbone_size8089
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BglII
promoterChicken bactin (plus Chicken bactin intron)
sequencing_primer_3 SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneNedd1
id299730
genbank_ids
mutationF75A in the CM1 domain
nameNedd1-mOrange2-F75A
shRNA_sequence
size898
species
10116
Rattus norvegicus
32644
Other
tags
plasmid copy :
pBeta-actin [Kapitein et al. , 2010]
resistance markers :
4675
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA