This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBactin-td-mCherry-Cdk5rap2-CTD
catalog :
196854
citations: 1
Reference
Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, et al. Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Neuron. 2023;111:1241-1263.e16 pubmed publisher
product information
Catalog Number :
196854
Product Name :
pBactin-td-mCherry-Cdk5rap2-CTD
article :
doi10.1016/j.neuron.2023.01.020
id28233695
pubmed_id36796357
bacterial resistance :
Ampicillin
cloning :
backbonepBeta-actin-AcGFP-Cdk5rap2-CTD
backbone_mutation
backbone_origin
backbone_size6513
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5NheI
promoterChicken bactin (plus Chicken bactin intron)
sequencing_primer_3 SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCdk5rap2
id286919
genbank_ids
mutationNone (wt)
nametd-mCherry-Cdk5rap2-CTD
shRNA_sequence
size1441
species
32644
Other
Discosoma sp.
tags
plasmid copy :
pBeta-actin [Kapitein et al. , 2010]
resistance markers :
4675
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA