This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOBD2 Pab1 WT (123-204)
catalog :
196834
citations: 1
Reference
Melamed D, YOUNG D, Miller C, Fields S. Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites. PLoS Genet. 2015;11:e1004918 pubmed publisher
product information
Catalog Number :
196834
Product Name :
pOBD2 Pab1 WT (123-204)
article :
doi10.1371/journal.pgen.1004918
id28233691
pubmed_id25671604
bacterial resistance :
Ampicillin
cloning :
backbonepOBD2
backbone_mutation
backbone_origin
backbone_size7167
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment. Expresses Pab1 fragment fused to GAL4BD.
growth strain :
Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NcoI
promoterADH
sequencing_primer_3
sequencing_primer_5Unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYER165W
genePAB1
id856912
genbank_ids
mutation123-204 amino acids
namePab1
shRNA_sequence
size241
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagGal4
resistance markers :
1441
tags :
Unknown
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA