This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOAD eIF4G1 (1-341)
catalog :
196833
citations: 1
Reference
Melamed D, YOUNG D, Miller C, Fields S. Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites. PLoS Genet. 2015;11:e1004918 pubmed publisher
product information
Catalog Number :
196833
Product Name :
pOAD eIF4G1 (1-341)
article :
doi10.1371/journal.pgen.1004918
id28233691
pubmed_id25671604
bacterial resistance :
Ampicillin
cloning :
backbonepOAD
backbone_mutation
backbone_origin
backbone_size8429
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
pOAD + EcoRI-eIF4G1(1-1023)-SalI. (W303 strain as a genomic template). Expresses eIF4G1 N' terminus fused to GAL4AD
growth strain :
Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterADH
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGR162W
geneTIF4631
id853071
genbank_ids
mutationamino acids 1-341
nameeIF4G1
shRNA_sequence
size1023
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagGal4
resistance markers :
1441
tags :
Unknown
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA