This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUGP1.1
catalog :
196615
citations: 1
Reference
Takhaveev V, xd6 zsezen S, Smith E, Zylstra A, Chaillet M, Chen H, et al. Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle. Nat Metab. 2023;5:294-313 pubmed publisher
product information
Catalog Number :
196615
Product Name :
pUGP1.1
article :
doi10.1038/s42255-023-00741-x
id28233659
pubmed_id36849832
bacterial resistance :
Ampicillin
cloning :
backbonepG23A, originally HO-poly-KanMX4-HO
backbone_mutation
backbone_originhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/
backbone_size2531
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Cloning details are provided in Supplementary Table 2 of the paper. Details of the parent plasmid pG23A can be found on Addgene (Plasmid #102884).
growth strain :
Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast
origin :
37
pi :
alt_names
YKL035W
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNative promoter
sequencing_primer_3CCTTGAAGCGCATGAACTC
sequencing_primer_5CTGTCAAGGAGGGTATTCTGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYKL035W
geneUGP1
id853830
genbank_ids
mutation
nameUGP1::mCherry-AID(71-114)-NatMX
shRNA_sequence
size3350
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagmCherry
locationC terminal on insert
tagAID(71-114)
resistance markers :
3411
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA