This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR
catalog :
196518
citations: 1
Reference
Calero Cuenca F, Osorio D, Carvalho Marques S, Sridhara S, Oliveira L, Jiao Y, et al. Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration. Curr Biol. 2021;31:1521-1530.e8 pubmed publisher
product information
Catalog Number :
196518
Product Name :
pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR
article :
doi10.1016/j.cub.2021.01.007
id28221824
pubmed_id33567288
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1+EeGFP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Contains attb1 and attb2 sites and a Linker sequence between Ctdnep1 and eGFP
growth strain :
Expression of Ctdnep1_D67E-GFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterCMV promoter; T7 promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDULLARD, HSA011916, NET56
geneCTDNEP1
id23399
genbank_ids
mutationD67E mutation (phosphastase dead). Silent mutations for siRNA resistance
nameCtdnep1_D67EsiR
shRNA_sequence
size799
species
9606
Homo sapiens
tags
locationC terminal on insert
tageGFP
resistance markers :
1620
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA