pSpCas9(BB)-2A-Puro G3BP1-sgRNA | Addgene 196197 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSpCas9(BB)-2A-Puro G3BP1-sgRNA

catalog :

196197

citations: 1

Reference
Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, D xfc rnberger G, et al. Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. elife. 2023;12: pubmed publisher

product information

Catalog Number :

196197

Product Name :

pSpCas9(BB)-2A-Puro G3BP1-sgRNA

article :

doi	10.7554/eLife.85135
id	28233587
pubmed_id	36989136

bacterial resistance :

Ampicillin

cloning :

backbone

PX459

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

growth notes :

Cloning was performed using pSpCas9(BB)-2A-GFP plasmid (Addgene, 48138, RRID:Addgene_48138), modified to express eCas9 instead of WT-Cas9 and dTomato instead of GFP (Esk et al., 2020), using the Bbs1 cloning strategy of Ran et al. (Ran et al., 2013).24 plasmid (Addgene, 48138, RRID:Addgene_48138), modified to express eCas9 instead of WT-25 Cas9 and dTomato instead of GFP (Esk et al., 2020), using the Bbs1 cloning strategy of Ran26 et al. (Ran et al., 2013). Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.

growth strain :

Cas9 + gRNA expression to edit G3BP1 locus

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	G3BP, HDH-VIII
gene	G3BP1
id	10146

genbank_ids

mutation

name

G3BP1

shRNA_sequence

unknown

size

species

9606

Homo sapiens