pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro | Addgene 196196 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

catalog :

196196

citations: 1

Reference
Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, D xfc rnberger G, et al. Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. elife. 2023;12: pubmed publisher

product information

Catalog Number :

196196

Product Name :

pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

article :

doi	10.7554/eLife.85135
id	28233587
pubmed_id	36989136

bacterial resistance :

Ampicillin

cloning :

backbone

pHDR_G3BP1-V5-APEX2-GFP

backbone_mutation

backbone_origin

Eugene Yeo; PubMed 29373831

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Homology repair donor

growth notes :

In Addgene vector 105284 the GFP was exchanged with the red fluorescent protein mScarlet-I using the plasmid pmScarlet-i_C1 (addgene 85044). Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.

growth strain :

homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	G3BP, HDH-VIII
gene	G3BP1
id	10146

genbank_ids

mutation

name

G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette

shRNA_sequence

size

species

9606

Homo sapiens

32644

Other