This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
catalog :
196191
citations: 1
Reference
Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, D xfc rnberger G, et al. Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. elife. 2023;12: pubmed publisher
product information
Catalog Number :
196191
Product Name :
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
article :
doi10.7554/eLife.85135
id28233587
pubmed_id36989136
bacterial resistance :
Ampicillin
cloning :
backbonepF2DTA
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Homology repair donor
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.
growth strain :
Tagging human SOX2 gene with P2A-EGFP at the C-terminus
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
mutationG2913A mutation to replace the guide cutting site in the Homology construct.
nameSOX2-HAL-P2A-EGFP-SOX2-HAR
shRNA_sequence
size
species
9606
Homo sapiens
32644
Other
tags
resistance markers :
6318
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA