This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pES002 MBP-GSK3 _S9A-HA-His
catalog :
196184
product information
Catalog Number :
196184
Product Name :
pES002 MBP-GSK3 _S9A-HA-His
article :
doi10.1101/2022.12.05.519208
id28233581
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepMBP-MG
backbone_mutationModified to include an N-terminal TEV-cleavable MBP tag and a C-terminal His6 tag
backbone_originNew England Biolabs
backbone_size6716
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2022.12.05.519208 for bioRxiv preprint.
growth strain :
Expresses MBP-GSK3 _S9A-HA-His (human Glycogen Synthase Kinase 3 S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli
origin :
37
pi :
alt_names
Glycogen Synthase Kinase 3
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterTac
sequencing_primer_3M13F Reverse TGTAAAACGACGGCCAGT
sequencing_primer_5MalE Forward GGTCGTCAGACTGTCGATGAAGCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneGSK3B
id2932
genbank_ids
2932
BC000251
mutationchanged Serine 9 to Alanine
nameGSK3B
shRNA_sequence
size1305
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMBP
locationC terminal on backbone
tagHis6
locationC terminal on insert
tagHA
plasmid copy :
Addgene #14753
resistance markers :
4035
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA