This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CAGGS-safb dd1-NLS-3XFLAG-V5
catalog :
196092
citations: 1
Reference
Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, et al. SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells. RNA. 2023;29:1535-1556 pubmed publisher
product information
Catalog Number :
196092
Product Name :
CAGGS-safb dd1-NLS-3XFLAG-V5
article :
doi10.1261/rna.079569.122
id28233571
pubmed_id37468167
bacterial resistance :
Ampicillin
cloning :
backboneAddgene Plasmid #196383
backbone_mutationInsertion of SAFB DD1 cDNA in AvrII and NotI sites.
backbone_originGenewiz
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth strain :
Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5AvrII
promoterCAGGS
sequencing_primer_3
sequencing_primer_5GGCTCTAGAGCCTCTGCTAAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases3110021E02Rik, 5330423C17Rik, E130307D12, HAP, HET, SAF-B1, SAFB1
geneSafb
id224903
genbank_ids
mutationRemoved amino acids 96-285 (predicted disordered domain 1) of mouse SAFB protein
nameSAFB
shRNA_sequence
size2277
species
10090
Mus musculus
tags
locationC terminal on backbone
tagC-terminal 3xFLAG
locationC terminal on backbone
tagC-terminal V5
plasmid copy :
Azenta (Genewiz)
resistance markers :
4461
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA