This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX SYT9 C2AB D197,199,330,332N
catalog :
195706
citations: 1
Reference
Seibert M, Evans C, Stanley K, Wu Z, Chapman E. Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion. J Neurosci. 2023;43:1475-1491 pubmed publisher
product information
Catalog Number :
195706
Product Name :
pGEX SYT9 C2AB D197,199,330,332N
article :
doi10.1523/JNEUROSCI.1857-22.2023
id28233200
pubmed_id36732068
bacterial resistance :
Ampicillin
cloning :
backbonepGEX
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2022.04.18.488681v2 for bioRxiv preprint.
growth strain :
Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations
growth temp :
Use BL21 for protein expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSytV
geneSyt5
id53420
genbank_ids
mutationencodes amino acids 104-386 of mouse SYT9 with D197, 199, 330, 332N mutations
nameSyt9
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on backbone
tagGST
resistance markers :
4536
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA