pcDNA5/FRT/TO V5 DNAJB3 | Addgene 19525 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA5/FRT/TO V5 DNAJB3

catalog :

19525

citations: 1

Reference
Hageman J, Kampinga H. Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. Cell Stress Chaperones. 2009;14:1-21 pubmed publisher

product information

Catalog Number :

19525

Product Name :

pcDNA5/FRT/TO V5 DNAJB3

article :

doi	10.1007/s12192-008-0060-2
id	2277
pubmed_id	18686016

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5137

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Part of the HSP40 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.

origin :

pi :

alt_names

DNAJB3

HCG3; MGC26879

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BamHI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	LNCX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HCG3, MGC26879
gene	DNAJB3
id	414061

genbank_ids

mutation

name

DnaJ (Hsp40) homolog, subfamily B, member 3

shRNA_sequence

size

782

species

10090

Mus musculus