pcDNA5/FRT/TO V5 HSPA8 | Addgene 19514 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA5/FRT/TO V5 HSPA8

catalog :

19514

citations: 8

Reference
Ikami Y, Terasawa K, Sakamoto K, Ohtake K, Harada H, Watabe T, et al. The two-domain architecture of LAMP2A regulates its interaction with Hsc70. Exp Cell Res. 2021;411:112986 pubmed publisher
Xu X, Sun Y, Cen X, Shan B, Zhao Q, Xie T, et al. Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model. Protein Cell. 2021;: pubmed publisher
Fu S, Hu M, Hsiao C, Cheng A, Chen T, Jeng C, et al. Regulation of ClC-2 Chloride Channel Proteostasis by Molecular Chaperones: Correction of Leukodystrophy-Associated Defect. Int J Mol Sci. 2021;22: pubmed publisher
Ryu S, Stewart R, Pectol D, Ender N, Wimalarathne O, Lee J, et al. Proteome-wide identification of HSP70/HSC70 chaperone clients in human cells. PLoS Biol. 2020;18:e3000606 pubmed publisher
Peng Y, Lee Y, Fu S, Chien Y, Liao Y, Chen T, et al. FKBP8 Enhances Protein Stability of the CLC-1 Chloride Channel at the Plasma Membrane. Int J Mol Sci. 2018;19: pubmed publisher
Kalogeropulou A, Zhao J, Bolliger M, Memou A, Narasimha S, Molitor T, et al. P62/SQSTM1 is a novel leucine-rich repeat kinase 2 (LRRK2) substrate that enhances neuronal toxicity. Biochem J. 2018;475:1271-1293 pubmed publisher
Peng Y, Huang J, Wu H, Hsieh H, Wu C, Chen S, et al. Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90?. Sci Rep. 2016;6:32444 pubmed publisher
Hageman J, Kampinga H. Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. Cell Stress Chaperones. 2009;14:1-21 pubmed publisher

product information

Catalog Number :

19514

Product Name :

pcDNA5/FRT/TO V5 HSPA8

article :

doi	10.1007/s12192-008-0060-2
id	2277
pubmed_id	18686016

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5137

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Part of the HSP70 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.

origin :

pi :

alt_names

HSPA8

LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10; MGC29929; MGC131511

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BamHI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	LNCX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
gene	HSPA8
id	3312

genbank_ids

mutation

name

heat shock 70kDa protein 8

shRNA_sequence

size

1994

species

9606

Homo sapiens