pcDNA5/FRT/TO V5 HSPA1A | Addgene 19510 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA5/FRT/TO V5 HSPA1A

catalog :

19510

citations: 5

Reference
Ryu S, Stewart R, Pectol D, Ender N, Wimalarathne O, Lee J, et al. Proteome-wide identification of HSP70/HSC70 chaperone clients in human cells. PLoS Biol. 2020;18:e3000606 pubmed publisher
Yu Y, Xia X, Li H, Zhang Y, Zhou X, Jiang H. A new rhodopsin R135W mutation induces endoplasmic reticulum stress and apoptosis in retinal pigment epithelial cells. J Cell Physiol. 2019;234:14100-14108 pubmed publisher
Kalogeropulou A, Zhao J, Bolliger M, Memou A, Narasimha S, Molitor T, et al. P62/SQSTM1 is a novel leucine-rich repeat kinase 2 (LRRK2) substrate that enhances neuronal toxicity. Biochem J. 2018;475:1271-1293 pubmed publisher
Peng Y, Huang J, Wu H, Hsieh H, Wu C, Chen S, et al. Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90?. Sci Rep. 2016;6:32444 pubmed publisher
Hageman J, Kampinga H. Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. Cell Stress Chaperones. 2009;14:1-21 pubmed publisher

product information

Catalog Number :

19510

Product Name :

pcDNA5/FRT/TO V5 HSPA1A

article :

doi	10.1007/s12192-008-0060-2
id	2277
pubmed_id	18686016

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5137

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Part of the HSP70 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.

origin :

pi :

alt_names

HSPA1A

HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BamHI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	LNCX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1
gene	HSPA1A
id	3303

genbank_ids

mutation

name

heat shock 70kDa protein 1A

shRNA_sequence

size

1979

species

9606

Homo sapiens